Juan J. Mercado, MD (neuropathology fellow at UAB 2015-17) |
A while ago, as a pathology resident, I was temporarily
in charge of organizing the weekly brain cutting activity. During this event I always felt a little bothered by the
unpredictability and irregularity that occurred with each cut and the
variability of results with each different person trying to pursue the same
goal. I
decided to do some research trying to find more information
about how brain grossing examination was
done in different places. To my amazement, I found out about a brain tissue bank in the United Kingdom that performed their coronal sections with the help of a
tool. This tool enabled them to create perfect
fine cuts every time to perform a complete meticulous evaluation. After knowing
about this, I was highly motivated to perform a DIY project. As I
optimistically anticipated, the results were excellent. I made the tool using materials
that I could easily find in any hardware store. This new and improved tool
could now be made by anyone interested in having the same results.
Materials:
·
Straight cabinet
handles:
they come in different diameters, meaning a different brain slice thickness can
be created depending on this diameter. Also they come in different lengths.
Choose a length proportionate to the size of the cutting board you select.
These bars always come with the screws included. (Fig. 2)
Figure 2 |
·
Drill: to make four holes
·
Rubber O-ring washers: not necessary, but
I use them just for the preservation of the tool, preventing liquids or tissue
to enter in the drilled holes (Fig. 3)
Figure 3 |
·
Rubber chair legs (to elevate the
board from a surface and to hold its placement) (Fig. 4)
Figure 4 |
With the above
materials you can create the basic version that will permit you to create cuts
of only one predetermined thickness based on the diameter of the bar you
select. You can also have an add-on to be able to do thinner cuts with the same
board, but it is not necessary.
Thanks, Dr. Mercado. I have often thought about how nice it would be to have a tool that could simplify and standardize braincutting. I am hoping he builds a limited-edition series of these devices and sells them at the next AANP meeting. Since I am not particularly mechanically inclined, I would be the first in line to purchase what I am hereby dubbing "The Mercado Brain Cutting Device"!
How
it works:
After detaching the
brainstem via an axial cut through the midbrain and then making the first brain
coronal section cut through the middle of the mammillary bodies, proceed as
usual making coronal sections but with the help of the tool
The bars aligned in the way
pictured (Fig. 5) serve to hold in place any brain size firmly while cutting.
Use a rigid knife sliding it above the bars as a guide. In this way the
thickness of the brain sections will be the same as the diameter of the bars
Figure 5 Advantages |
·
Always
the same results, not relying on the experience of the person cutting the brain -- meaning standardization.
·
Homogeneous
leveled slices. Option of creating thin slices help in a more meticulous
evaluation.
·
When
a pathologic finding is present, such as a big intraparenchymal hemorrhage that
normally disintegrates the brain slice if performed by normal technique; it does
not happen with this tool.
·
Better
pictures.
·
It
is a lot faster and the cuts are perfect. Less time cutting, more
learning and teaching.
See for yourself…
Add-on(s):
Optional (need a saw)
A thinner cutting
board cut to fit within the two bars. The diameter of the bar minus the
thickness of this board will be your new brain slice thickness, making the same
board practical for two different thicknesses.
Thanks, Dr. Mercado. I have often thought about how nice it would be to have a tool that could simplify and standardize braincutting. I am hoping he builds a limited-edition series of these devices and sells them at the next AANP meeting. Since I am not particularly mechanically inclined, I would be the first in line to purchase what I am hereby dubbing "The Mercado Brain Cutting Device"!
4 comments:
Actually, I did my whole residency with a version of this. It Is good to have a guide for regular even slices. I trained in Canada and I think these are standard there, so much so that when I moved to Boston, I had my own custom-made. I find my version more suited to me, as they are transportable and you just place the guides on the cutting board (without screws and tools, etc) and you can move them if necessary
This doesn't seem to allow me to attach a picture but they are basically a square frame of
stainless steel (2 square L-shaped pieces) one cm thick, that you take with you to brain-cutting.. or a dinner party..
As I say, I trained 15 years ago thinking these were quite standard but they were nowhere to be found in America, and googling brain-cutting guides was not productive
oh, actually so common and necessary were these guides, that they came in two formats
based on your personal preference. The cylindrical "rollers" as i think is depicted here,
or the straight flat guides, which i use.
Thid brain cutting board is so brilliant, I tried to Google it but I didn't find it anywhere, Do you know where can I buy one or get one online? thank you
Not sure where you might be able to obtain one. Perhaps email Dr. Mercado at Alabama to see if he can help you..... Good luck.
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