tag:blogger.com,1999:blog-5424255638293718915.post49356969280848534..comments2024-03-18T01:10:51.745-05:00Comments on neuropathology blog: D. T. Max writes the back story on prion diseaseBrian E. Moore, MD, MEdhttp://www.blogger.com/profile/17503916201692804693noreply@blogger.comBlogger6125tag:blogger.com,1999:blog-5424255638293718915.post-70804085315932625472013-04-24T13:19:52.686-05:002013-04-24T13:19:52.686-05:00say there dt, an update for you. hope you and your...say there dt, an update for you. hope you and yours are well...<br /><br /><br /><br /><br />Monday, April 22, 2013 <br /><br />North Carolina Senate bill S.B. 648 could be health risk and risk your children again to mad cow type disease BSE TSE prion disease <br /><br />Letter: Senate bill could be health risk <br /><br />http://downercattle.blogspot.com/2013/04/north-carolina-senate-bill-sb-648-could.html<br /><br /><br /><br /><br /> <br />Wednesday, April 24, 2013 <br /> <br />Dissociation between Transmissible Spongiform Encephalopathy (TSE) Infectivity and Proteinase K-Resistant PrPSc Levels in Peripheral Tissue from a Murine Transgenic Model of TSE Disease<br /> <br />http://transmissiblespongiformencephalopathy.blogspot.com/2013/04/dissociation-between-transmissible.html<br /> <br /> <br /> <br /> <br /> <br /><br /><br /><br />kind regards,<br />flatfishTerry S. Singeltary Sr.https://www.blogger.com/profile/06986622967539963260noreply@blogger.comtag:blogger.com,1999:blog-5424255638293718915.post-89344284029601890752012-02-06T11:21:47.682-06:002012-02-06T11:21:47.682-06:00In addition, non-human primates are specifically s...In addition, non-human primates are specifically susceptible for atypical BSE as demonstrated by an approximately 50% shortened incubation time for L-type BSE as compared to C-type. Considering the current scientific information available, it cannot be assumed that these different BSE types pose the same human health risks as C-type BSE or that these risks are mitigated by the same protective measures. <br /> <br /> <br /> <br />http://www.prionetcanada.ca/detail.aspx?menu=5&dt=293380&app=93&cat1=387&tp=20&lk=no&cat2 <br /> <br /> <br /> <br />Thursday, August 12, 2010<br /> <br />Seven main threats for the future linked to prions<br /> <br />First threat<br /> <br />***Also, a link is suspected between atypical BSE and some apparently sporadic cases of Creutzfeldt-Jakob disease in humans. These atypical BSE cases constitute an unforeseen first threat that could sharply modify the European approach to prion diseases.<br /> <br /><br />Second threat <br /> <br />snip... <br /> <br /><br /> http://www.neuroprion.org/en/np-neuroprion.html <br /> <br /><br /> <br /> <br />Monday, October 10, 2011 <br /> <br />EFSA Journal 2011 The European Response to BSE: A Success Story <br /> <br />snip... <br /><br /><br />This opinion confirmed Classical BSE prions as the only TSE agents demonstrated to be zoonotic so far but the possibility that a small proportion of human cases so far classified as "sporadic" CJD are of zoonotic origin could not be excluded. Moreover, transmission experiments to non-human primates suggest that some TSE agents in addition to Classical BSE prions in cattle (namely L-type Atypical BSE, Classical BSE in sheep, transmissible mink encephalopathy (TME) and chronic wasting disease (CWD) agents) might have zoonotic potential. <br /> <br />snip... <br /> <br />http://www.efsa.europa.eu/en/efsajournal/pub/e991.htm?emt=1 <br /> <br />http://www.efsa.europa.eu/en/efsajournal/doc/e991.pdf <br /> <br /><br />see follow-up here about North America BSE Mad Cow TSE prion risk factors, and the ever emerging strains of Transmissible Spongiform Encephalopathy in many species here in the USA, including humans ; <br /> <br /> <br /> <br />http://transmissiblespongiformencephalopathy.blogspot.com/2011/10/efsa-journal-2011-european-response-to.html <br /> <br /> <br /> <br /><br /><br /> <br />Sunday, February 5, 2012<br /> <br /> <br />February 2012 Update on Feed Enforcement Activities to Limit the Spread of BSE <br /> <br /> <br />http://transmissiblespongiformencephalopathy.blogspot.com/2012/02/february-2012-update-on-feed.html<br /> <br /> <br /> <br /> <br /><br /><br /><br /> <br />Thursday, January 26, 2012<br /> <br />Facilitated Cross-Species Transmission of Prions in Extraneural Tissue <br /> <br /> <br /><br /><br />Science 27 January 2012: <br />Vol. 335 no. 6067 pp. 472-475 <br />DOI: 10.1126/science.1215659 <br /><br /><br /> <br /> <br />http://transmissiblespongiformencephalopathy.blogspot.com/2012/01/facilitated-cross-species-transmission.html<br /> <br /> <br /> <br /> <br /><br />TSSTerry S. Singeltary Sr.https://www.blogger.com/profile/06986622967539963260noreply@blogger.comtag:blogger.com,1999:blog-5424255638293718915.post-23874941502953647882009-06-01T14:06:56.656-05:002009-06-01T14:06:56.656-05:00From Dr. Doug Miller, neuropathologist at Universi...From Dr. Doug Miller, neuropathologist at University of Missouri:<br /><br />1) Is there sporadic CJD? Well, there is little to no evidence for CJD as an “infectious” disease as compared to a “transmissible” disease. Transmission as one spreads a cold or flu or other infectious disease from person to person is extremely rare, virtually not documented. Most cases are isolated single cases, clusters are very unusual and most of those reported have a genetic basis. There is nothing to suggest that prior to the UK BSE epidemic that there was a hidden epidemic of CJD in the human population, and incidence rates have not changed in the USA or other countries, cCJD aside, since the BSE epidemic, to my knowledge.<br /><br />2) The assertion that because the “agent” (I like “prion”, but if some don’t, OK) is not inactivated by formalin fixation does not mean it remains “active” in our tissue sections. I have CJD recut slides from autopsies from my residency back in 1982 and 1983 in my slide collection, and I recently photographed a few of those for a lecture…and can compare those photomicrographs to some of the same cases taken on film back in 1982, 83, or 84. There has not been “in vitro” progression of the disease since then. The suggestion that CJD continues to destroy brain tissue under a cover slip after fixation, dehydration, paraffin infiltration and embedding, sectioning, and staining is ludicrous, mainly because it is against a huge experience we neuropathologists all have. Sure, if you take off the cover slip, scrape off the tissue, grind it up, and inject it into a susceptible animal (or person) I have no doubt you can transmit CJD, but that’s not the same thing as the person who commented on your blog stated. I urge any neuropathologist with evidence to the contrary to come forward but I am sure it won’t happen because I have slides, and have seen many others, and they don’t show this supposed progressive change.Brian E. Moore, MD, MEdhttps://www.blogger.com/profile/17503916201692804693noreply@blogger.comtag:blogger.com,1999:blog-5424255638293718915.post-25071450067025742312009-05-10T12:32:00.000-05:002009-05-10T12:32:00.000-05:00Interesting Dr. MooreInteresting Dr. MooreAdam Kinghttps://www.blogger.com/profile/12888814773419809679noreply@blogger.comtag:blogger.com,1999:blog-5424255638293718915.post-35103048040028574722009-05-10T10:39:00.000-05:002009-05-10T10:39:00.000-05:00You sure got a long comment on this one!You sure got a long comment on this one!jdnoreply@blogger.comtag:blogger.com,1999:blog-5424255638293718915.post-63353345607978773392009-05-08T10:15:00.000-05:002009-05-08T10:15:00.000-05:00re- D.T. Max comments on Sporadic CJD ;
''...re- D.T. Max comments on Sporadic CJD ;<br /><br />''Some patient advocacy groups claim that CJD cases now classified as sporadic are in fact infectious. "A surprising number of mainstream scientists also doubt the existence of sporadic CJD''<br /><br /><br />amen, thank you there D.T. !!!<br /><br /><br />also, IF any one out thinks that the largest U.S. beef recall in the U.S. was because a few animals were abused, well, you are wrong. fact is, the USDA certified dead stock downer cow school lunch program exposed our children all across the U.S. to the most highest risk cattle to mad cow disease i.e. 'downer cows'. who will watch the children in the years, decades to come, for CJD? how many exposed, will be silent carriers of second hand transmission i.e. 'friendly fire' ? only time will tell, but as long as society continues to accept this term 'sporadic' and or 'spontaneous', as an answer, as opposed to an excuse, then we will never know, and that's their whole damn purpose $$$<br /><br />TSS<br /><br /><br /><br />IN REPLY TO ;<br /><br />FOR IMMEDIATE RELEASE Friday, May 1, 2009 WWW.USDOJ.GOVCIV (202) 514-2007 TDD (202) 514-1888<br /><br />U.S. Intervenes in Suit Against Former Beef Suppliers to National School Lunch Program<br /><br />Inhumane Treatment and Slaughter of Disabled, Non-Ambulatory Cattle at Issue WASHINGTON -- The United States has intervened in a civil lawsuit against two former suppliers to the National School Lunch Program – Hallmark Meat Packing Company and Westland Meat Company Inc. – for submitting false and fraudulent claims to the Agricultural Marketing Service (AMS), a division of the U.S. Department of Agriculture (USDA), the Justice Department announced today. All ground beef containing defendants’ products was recalled by USDA as of Feb. 16, 2008, and defendants no longer supply beef to the National School Lunch Program or AMS.<br /><br />The National School Lunch Program is a federally-assisted meal program operating in public and nonprofit private schools and residential child care institutions. The program, established under the National School Lunch Act in 1946, provides nutritionally balanced, low-cost or free lunches to children each school day.<br /><br />The suit was originally filed by the Humane Society of the United States under the qui tam or whistleblower provisions of the False Claims Act (FCA). In its complaint, the Humane Society alleges that defendants knowingly and falsely represented to AMS that all cattle at their slaughtering facility are humanely handled in accordance with federal regulations and that no meat from disabled, non-ambulatory cattle was included in AMS’ purchases.<br /><br />"The alleged misrepresentations by Hallmark and Westland could have impacted the health of many of our nation’s most vulnerable citizens--our schoolchildren," said Tony West, Assistant Attorney General of the Justice Department’s Civil Division. "Our intervention in this case demonstrates how seriously we will pursue allegations such as these."<br /><br />Under qui tam statute, a private party, known as a "relator," can file an action on behalf of the United States and receive a portion of the recovery. Under the FCA, the government is entitled to treble damages plus civil penalties ranging from $5,500 to $11,000 per violation.<br /><br />The Department’s Civil Division and the U.S. Attorney’s Office for the Central District of California will pursue the case. The government plans to file an amended complaint. The U.S. Department of Agriculture’s Office of Inspector General investigated the matter.<br /><br />###<br /><br />09-426<br /><br /><br />http://www.usdoj.gov/opa/pr/2009/May/09-civ-426.html<br /><br /><br />Saturday, March 14, 2009 Agriculture Secretary Tom Vilsack Announces Final Rule for Handling of Non-Ambulatory Cattle<br /><br /><br />http://www.usda.gov/2009/03/0060.xml<br /><br /><br />THANK YOU !!!<br /><br />THANK GOD ! after years and years of exposing, especially our children with dead stock downer cows, from the USDA et al dead stock downer cow school lunch program, finally, some common sense comes forth. ...TSS<br /><br />DEADSTOCK DOWNER CATTLE THE MOST HIGH RISK FOR MAD COW DISEASE, and the USDA et al have been force feeding your children this for years. who will monitor our children in the years, decades to come for CJD aka mad cow disease ??? SCHOOL LUNCH PROGRAM FROM DOWNER CATTLE UPDATE IS THERE A SCRAPIE-LIKE DISEASE IN CATTLE ? In April of 1985, a mink rancher in Wisconsin reported a debilitating neurologic disease in his herd which we diagnosed as TME by histopathologic findings confirmed by experimental transmission to mink and squirrel monkeys. The rancher was a ''dead stock'' feeder using mostly (>95%) downer or dead dairy cattle and a few horses. She had never been fed. We believe that these findings may indicate the presence of a previously unrecognized scrapie-like disease in cattle and wish to alert dairy practitioners to this possibility. snip... PROCEEDINGS OF THE SEVENTH ANNUAL WESTERN CONFERENCE FOR FOOD ANIMAL VETERINARY MEDICINE, University of Arizona, March 17-19, 1986<br /><br /><br />http://www.bseinquiry.gov.uk/files/mb/m09a/tab01.pdf<br /><br /><br />http://www.bseinquiry.gov.uk/files/mb/m09/tab05.pdf<br /><br /><br />IS THERE A SCRAPIE-LIKE DISEASE IN CATTLE ? YOU BET THERE IS, AND HAS BEEN, AND WE BEEN FEEDING THE MOST HIGH RISK I.E. DEAD STOCK DOWNER COWS TO OUR CHILDREN FOR DECADES, who will follow these children for human TSE from mad cow disease here in the USA in the years, decades to come, and how many will they expose from the 'pass it forward' friendly fire modes ???<br /><br /><br />http://downercattle.blogspot.com/2008/12/evaluation-of-fsis-management-controls.html<br /><br /><br /><br />http://downercattle.blogspot.com/<br /><br /><br />Wednesday, February 11, 2009 Atypical BSE North America Update February 2009 Both of the BSE cases ascertained in the US native-born cattle were atypical cases (H-type), which contributed to the initial ambiguity of the diagnosis. 174, 185 In Canada, there have been 2 atypical BSE cases in addition to the 14 cases of the classic UK strain of BSE2: one was the H-type, and the other was of the L-type.198 snip...end source : Enhanced Abstract Journal of the American Veterinary Medical Association January 1, 2009, Vol. 234, No. 1, Pages 59-72 Bovine spongiform encephalopathy Jane L. Harman, DVM, PhD; Christopher J. Silva, PhD<br /><br /><br />http://avmajournals.avma.org/doi/ref/10.2460/javma.234.1.59<br /><br /><br />Atypical BSE North America Update February 2009<br /><br /><br />http://bse-atypical.blogspot.com/2009/02/atypical-bse-north-america-update.html<br /><br /><br />Thursday, April 30, 2009 FDA Issues Final Guidance for Renderers on Substances Prohibited From Use in Animal Food or Feed CVM Update Back April 30, 2009<br /><br /><br />http://madcowfeed.blogspot.com/2009/04/fda-issues-final-guidance-for-renderers.html<br /><br /><br />April 20, 2009 National Prion Disease Pathology Surveillance Center Cases Examined1 (December 31, 2008) National Prion Disease Pathology Surveillance Center Cases Examined1 (December 31, 2008) Year Total Referrals2 Prion Disease Sporadic Familial Iatrogenic vCJD 1996 & earlier 42 32 28 4 0 0 1997 115 68 59 9 0 0 1998 93 53 45 7 1 0 1999 115 69 61 8 0 0 2000 151 103 89 14 0 0 2001 210 118 108 9 0 0 2002 258 147 123 22 2 0 2003 273 176 135 41 0 0 2004 335 184 162 21 0 13 2005 346 193 154 38 1 0 2006 380 192 159 32 0 14 2007 370 212 185 26 0 0 2008 383 228 182 23 0 0 TOTAL 30715 17756 1490 254 4 2 1 Listed based on the year of death or, if not available, on year of referral; 2 Cases with suspected prion disease for which brain tissue and/or blood (in familial cases) were submitted; 3 Disease acquired in the United Kingdom; 4 Disease acquired in Saudi Arabia; 5 Includes 20 cases in which the diagnosis is pending, and 17 inconclusive cases; 6 Includes 25 cases with type determination pending in which the diagnosis of vCJD has been excluded. Rev 2/13/09 National<br /><br /><br />http://www.cjdsurveillance.com/pdf/case-table.pdf<br /><br /><br />http://www.cjdsurveillance.com/resources-casereport.html<br /><br /><br />http://www.aan.com/news/?event=read&article_id=4397&page=72.45.45<br /><br /><br />*5 Includes 20 cases in which the diagnosis is pending, and 17 inconclusive cases; *6 Includes 25 cases with type determination pending in which the diagnosis of vCJD has been excluded. Greetings, it would be interesting to know what year these atypical cases occurred, as opposed to lumping them in with the totals only. are they accumulating ? did they occur in one year, two years, same state, same city ? location would be very interesting ? age group ? sex ? how was it determined that nvCJD was ruled out ? from 1997, the year i started dealing with this nightmare, there were 28 cases (per this report), up until 2007 where the total was 185 cases (per this report), and to date 2008 is at 182. a staggering increase in my opinion, for something that just happens spontaneously as some would have us believe. i don't believe it, not in 85%+ of all sporadic CJD cases. actually, i do not believe yet that anyone has proven that any of the sporadic CJD cases have been proven to be a spontaneous misfolding of a protein. there are many potential routes and sources for the sporadic CJD's. ...TSS Sunday, April 12, 2009 r-calf and the USA mad cow problem, don't look, don't find, and then blame Canada<br /><br /><br />http://prionunitusaupdate2008.blogspot.com/2009/04/r-calf-and-usa-mad-cow-problem-dont.html<br /><br /><br />snip...SEE FULL TEXT BELOW ! Monday, April 20, 2009 National Prion Disease Pathology Surveillance Center Cases Examined1 (December 31, 2008)<br /><br /><br />http://prionunitusaupdate2008.blogspot.com/2009/04/national-prion-disease-pathology.html<br /><br /><br /><br />Friday, November 30, 2007<br /><br />CJD QUESTIONNAIRE USA CWRU AND CJD FOUNDATION<br /><br />snip...<br /><br />i am reminded of a few things deep throat told me years ago;<br /><br /><br />=================================================2001<br /><br /><br />The most frightening thing I have read all day is the report of Gambetti's finding of a new strain of sporadic cjd in young people......... Dear God, what in the name of all that is holy is that!!! If the US has different strains of scrapie..... why???? than the UK... then would the same mechanisms that make different strains of scrapie here make different strains of BSE... if the patterns are different in sheep and mice for scrapie..... could not the BSE be different in the cattle, in the mink, in the humans....... I really think the slides or tissues and everything from these young people with the new strain of sporadic cjd should be put up to be analyzed by many, many experts in cjd........ bse..... scrapie<br /><br />Scrape the damn slide and put it into mice..... wait..... chop up the mouse brain and and spinal cord........ put into some more mice..... dammit amplify the thing and start the damned research..... This is NOT rocket science... we need to use what we know and get off our butts and move.... the whining about how long everything takes..... well it takes a whole lot longer if you whine for a year and then start the research!!!<br /><br />Not sure where I read this but it was a recent press release or something like that: I thought I would fall out of my chair when I read about how there was no worry about infectivity from a histopath slide or tissues because they are preserved in formic acid, or formalin or formaldehyde..... for God's sake........ Ask any pathologist in the UK what the brain tissues in the formalin looks like after a year....... it is a big fat sponge... the agent continues to eat the brain ...... you can't make slides anymore because the agent has never stopped........ and the old slides that are stained with Hemolysin and Eosin...... they get holier and holier and degenerate and continue... what you looked at 6 months ago is not there........ Gambetti better be photographing every damned thing he is looking at.....<br /><br />Okay, you need to know. You don't need to pass it on as nothing will come of it and there is not a damned thing anyone can do about it. Don't even hint at it as it will be denied and laughed at.......... USDA is gonna do as little as possible until there is actually a human case in the USA of the nvcjd........ if you want to move this thing along and shake the earth.... then we gotta get the victims families to make sure whoever is doing the autopsy is credible, trustworthy, and a saint with the courage of Joan of Arc........ I am not kidding!!!! so, unless we get a human death from EXACTLY the same form with EXACTLY the same histopath lesions as seen in the UK nvcjd........ forget any action........ it is ALL gonna be sporadic!!! And, if there is a case....... there is gonna be every effort to link it to international travel, international food, etc. etc. etc. etc. etc. They will go so far as to find out if a sex partner had ever traveled to the UK/europe, etc. etc. .... It is gonna be a long, lonely, dangerous twisted journey to the truth. They have all the cards, all the money, and are willing to threaten and carry out those threats.... and this may be their biggest downfall...<br /><br />Thanks as always for your help. (Recently had a very startling revelation from a rather senior person in government here.......... knocked me out of my chair........ you must keep pushing. If I was a power person.... I would be demanding that there be at least a million bovine tested as soon as possible and agressively seeking this disease. The big players are coming out of the wood work as there is money to be made!!!<br /><br />In short: "FIRE AT WILL"!!! for the very dumb.... who's "will"! "Will be the burden to bare if there is any coverup!"<br /><br />again it was said years ago and it should be taken seriously.... BSE will NEVER be found in the US!<br /><br />As for the BSE conference call... I think you did agreat service to freedom of information and making some people feign integrity... I find it scary to see that most of the "experts" are employed by the federal government or are supported on the "teat" of federal funds. A scary picture! I hope there is a confidential panel organized by the new government to really investigate this thing.<br /><br />You need to watch your back........ but keep picking at them....... like a buzzard to the bone... you just may get to the truth!!! (You probably have more support than you know. Too many people are afraid to show you or let anyone else know. I have heard a few things myself... you ask the questions that everyone else is too afraid to ask.)<br /><br /><br />================================================<br /><br /><br />greetings again voice,<br /><br />then i remind everyone to read this;<br /><br />'As implied in the Inset 25 we must not assume that transmission of BSE to other species will invariably present pathology typical of a scrapie-like disease.'<br /><br /><br />http://www.bseinquiry.gov.uk/files/yb/1991/01/04004001.pdf<br /><br /><br />CWRU CJD QUESTIONNAIRE HISTORY<br /><br /><br />http://cjdquestionnaire.blogspot.com/<br /><br /><br />Terry S. Singeltary Sr. P.O. Box 42 Bacliff, Texas USA 77518<br /><br />Saturday, May 2, 2009<br /><br />U.S. GOVERNMENT SUES WESTLAND/HALLMARK MEAT OVER USDA CERTIFIED DEADSTOCK DOWNER COW SCHOOL LUNCH PROGRAM<br /><br /><br />http://downercattle.blogspot.com/2009/05/us-government-sues-westlandhallmark.html<br /><br /><br />USA MAD COW TESTING (or the lack of)<br /><br />Sunday, April 12, 2009<br /><br />BSE MAD COW TESTING USA 2009 FIGURES<br /><br />Month Number of Tests<br /><br />Feb 2009 -- 1,891<br /><br />Jan 2009 -- 4,620<br /><br /><br />http://www.aphis.usda.gov/newsroom/hot_issues/bse/surveillance/ongoing_surv_results.shtml<br /><br /><br />http://madcowtesting.blogspot.com/2008/04/mbm-greaves-meat-offal-live-cattle.html<br /><br /><br />P02.35<br /><br />Molecular Features of the Protease-resistant Prion Protein (PrPres) in H-type BSE<br /><br />Biacabe, A-G1; Jacobs, JG2; Gavier-Widén, D3; Vulin, J1; Langeveld, JPM2; Baron, TGM1 1AFSSA, France; 2CIDC-Lelystad, Netherlands; 3SVA, Sweden<br /><br />Western blot analyses of PrPres accumulating in the brain of BSE-infected cattle have demonstrated 3 different molecular phenotypes regarding to the apparent molecular masses and glycoform ratios of PrPres bands. We initially described isolates (H-type BSE) essentially characterized by higher PrPres molecular mass and decreased levels of the diglycosylated PrPres band, in contrast to the classical type of BSE. This type is also distinct from another BSE phenotype named L-type BSE, or also BASE (for Bovine Amyloid Spongiform Encephalopathy), mainly characterized by a low representation of the diglycosylated PrPres band as well as a lower PrPres molecular mass. Retrospective molecular studies in France of all available BSE cases older than 8 years old and of part of the other cases identified since the beginning of the exhaustive surveillance of the disease in 20001 allowed to identify 7 H-type BSE cases, among 594 BSE cases that could be classified as classical, L- or H-type BSE. By Western blot analysis of H-type PrPres, we described a remarkable specific feature with antibodies raised against the C-terminal region of PrP that demonstrated the existence of a more C-terminal cleaved form of PrPres (named PrPres#2 ), in addition to the usual PrPres form (PrPres #1). In the unglycosylated form, PrPres #2 migrates at about 14 kDa, compared to 20 kDa for PrPres #1. The proportion of the PrPres#2 in cattle seems to by higher compared to the PrPres#1. Furthermore another PK-resistant fragment at about 7 kDa was detected by some more N-terminal antibodies and presumed to be the result of cleavages of both N- and C-terminal parts of PrP. These singular features were maintained after transmission of the disease to C57Bl/6 mice. The identification of these two additional PrPres fragments (PrPres #2 and 7kDa band) reminds features reported respectively in sporadic Creutzfeldt-Jakob disease and in Gerstmann-Sträussler-Scheinker (GSS) syndrome in humans.<br /><br /><br />http://www.neuroprion.com/pdf_docs/conferences/prion2007/abstract_book.pdf<br /><br /><br />Research Project: Study of Atypical Bse Location: Virus and Prion Diseases of Livestock<br /><br />Project Number: 3625-32000-086-05 Project Type: Specific Cooperative Agreement<br /><br />Start Date: Sep 15, 2004 End Date: Sep 14, 2009<br /><br />Objective: The objective of this cooperative research project with Dr. Maria Caramelli from the Italian BSE Reference Laboratory in Turin, Italy, is to conduct comparative studies with the U.S. bovine spongiform encephalopathy (BSE) isolate and the atypical BSE isolates identified in Italy. The studies will cover the following areas: 1. Evaluation of present diagnostics tools used in the U.S. for the detection of atypical BSE cases. 2. Molecular comparison of the U.S. BSE isolate and other typical BSE isolates with atypical BSE cases. 3. Studies on transmissibility and tissue distribution of atypical BSE isolates in cattle and other species.<br /><br />Approach: This project will be done as a Specific Cooperative Agreement with the Italian BSE Reference Laboratory, Istituto Zooprofilattico Sperimentale del Piemonte, in Turin, Italy. It is essential for the U.S. BSE surveillance program to analyze the effectiveness of the U.S diagnostic tools for detection of atypical cases of BSE. Molecular comparisons of the U.S. BSE isolate with atypical BSE isolates will provide further characterization of the U.S. BSE isolate. Transmission studies are already underway using brain homogenates from atypical BSE cases into mice, cattle and sheep. It will be critical to see whether the atypical BSE isolates behave similarly to typical BSE isolates in terms of transmissibility and disease pathogenesis. If transmission occurs, tissue distribution comparisons will be made between cattle infected with the atypical BSE isolate and the U.S. BSE isolate. Differences in tissue distribution could require new regulations regarding specific risk material (SRM) removal.<br /><br /><br />http://www.ars.usda.gov/research/projects/projects.htm?ACCN_NO=408490<br /><br /><br />Wednesday, February 11, 2009<br /><br />Atypical BSE North America Update February 2009<br /><br />Both of the BSE cases ascertained in the US native-born cattle were atypical cases (H-type), which contributed to the initial ambiguity of the diagnosis. 174, 185 In Canada, there have been 2 atypical BSE cases in addition to the 14 cases of the classic UK strain of BSE2: one was the H-type, and the other was of the L- type.198<br /><br />snip...end<br /><br />source :<br /><br />Enhanced Abstract Journal of the American Veterinary Medical Association January 1, 2009, Vol. 234, No. 1, Pages 59-72<br /><br />Bovine spongiform encephalopathy<br /><br />Jane L. Harman, DVM, PhD; Christopher J. Silva, PhD<br /><br /><br />http://avmajournals.avma.org/doi/ref/10.2460/javma.234.1.59<br /><br /><br />Atypical BSE North America Update February 2009<br /><br /><br />http://bse-atypical.blogspot.com/2009/02/atypical-bse-north-america-update.html<br /><br /><br />Thursday, April 9, 2009<br /><br />Docket No. FDA2002N0031 (formerly Docket No. 2002N0273) RIN 0910AF46 Substances Prohibited From Use in Animal Food or Feed; Final Rule: Proposed<br /><br /><br />http://madcowfeed.blogspot.com/2009/04/docket-no-fda2002n0031-formerly-docket.html<br /><br /><br />full text ;<br /><br />Sunday, April 12, 2009 TRANSMISSION OF ATYPICAL BOVINE SPONGIFORM ENCEPHALOPATHY (BSE) IN HUMANIZED MOUSE MODELS<br /><br /><br />http://bse-atypical.blogspot.com/2009/04/transmission-of-atypical-bovine.html<br /><br /><br />Thursday, April 9, 2009<br /><br />Docket No. FDA2002N0031 (formerly Docket No. 2002N0273) RIN 0910AF46 Substances Prohibited From Use in Animal Food or Feed; Final Rule: Proposed<br /><br /><br />http://madcowfeed.blogspot.com/2009/04/docket-no-fda2002n0031-formerly-docket.html<br /><br /><br /><br />http://prionunitusaupdate2008.blogspot.com/2009/04/r-calf-and-usa-mad-cow-problem-dont.html#comments<br /><br /><br />Sunday, April 12, 2009 r-calf and the USA mad cow problem, don't look, don't find, and then blame Canada<br /><br /><br /><br />http://prionunitusaupdate2008.blogspot.com/2009/04/r-calf-and-usa-mad-cow-problem-dont.html<br /><br /><br />http://prionunitusaupdate2008.blogspot.com/2009/04/cjd-foundation-sides-with-r-calfers-no.html#comments<br /><br /><br />Terry S. Singeltary Sr. <br />P.O. Box 42 <br />Bacliff, Texas USA 77518Anonymousnoreply@blogger.com